This manual is the first section of a three part leica tcs sp5 user guide. The leica sp5 laser scanning confocal system is used for advanced multichannel fluorescence and livecell applications. With an intuitive user interface and straightforward navigation, it guides the user through any workflow, whether fast image acquisition or sophisticated expert analysis. National chiao tung university, bio photonics lab, bypraveen. How can i add the scale bar to an image acquired with. Leica sp5 ii laser scanning confocal microscope biological.
Leica tcs sp5 ii acoustooptical beam splitter products. Confocal microscope system with a broad range of scan speeds suited for highresolution. This new broadband confocal microscope system perfectly merges two worlds in one. How to use leica confocal microscope sp5 external laser.
Leica sp5 laser scanning confocal microscope system. Introduction to confocal laser scanning microscopy leica. Leica tcs sp5 confocal microscopes products home leica leica microsystems. Leica sp5 confocal microscope department of molecular biology. Scanning with confocal using the leica tcs sp5 confocal microscope. How can i add the scale bar to an image acquired with leica sp5 confocal microscope using imagej. Instead of using mirrors to raster a single laser beam across the. Leica sp5 scanning confocal cdb equipment resource. Leica dm6000 cfs upright multiphoton microscope equipped with a tcs sp5 mp laser confocal with both galvonomic and resonance scanners and with spectral hyd detectors. Leica sp5 confocal microscope four laser uv, argon, dpss, hene. Leica sp5 inverted confocal duke light microscopy core. Leica sp5 laser scanning confocal microscope ucsf drc. This is a very flexible and fast confocal system build with a leica dmi 6000 cs inverted, fully automated microscope with motorized.
This 45 minute video is the basic training of the use of the leica sp5 confocal microscope in the mbf. The system provides the full range of scan speeds at the highest resolution. This confocal is reserved for primary and joint cdb faculty and their personnel. Open source free software for a leica tcs sp5 confocal. The leica tcs sp5 mp covers a wide range of imaging applications multiphoton and one photon by combining two technologies in one system. In the acquire tab, choose a saved or preset laser beam setting in the leica. The white light laserbased sp5x system is a significant advance in confocal microscopy. Leica sp5 tile scan protocol icahn school of medicine. A spinning disk confocal microscope differs slightly from a lscm in how it forms the image. This system has five lasers generating nine lines for fluorescence excitation 405. To maintain information on the most current level, the description of software functions. Open the leica confocal las af software it takes a few. The leica sp5 confocal fully covers a broad range of requirements in confocal and multiphoton imaging with excellent overall performance. Select the objective you want to use either inside software or on microscope ctrl panel do not fysically.
Fluorescence lifetime imaging was performed on the penn vet imaging cores leica sp5 ii inverted confocalflim microscope with a 63x water immersion lens by scanning the sample with a 405 nm. The leica tcs sp5 mp covers a wide range of imaging applica tions multiphoton and one photon by combining two technolo gies in one system. Imaging diverse fluorophores in fixed or live samples. How to use leica confocal microscope sp5 external laser part1. Laser point scan confocal with standard and highspeed resonance scanning. Leica dmi6000cs inverted live cell chamber ludin cube metal halide fluorescence source motorized scanning stage tandem scanners select at software initialization conventional. Confocal microscopes confocal microscopes from leica microsystems are partners in top level biomedical research and surface analysis in material science applications, offering unprecedented. Leica sp5 x confocalspectral imaging microscopy system with white light laser. Microscope imaging software products leica microsystems. The leica tcs sp5 confocal fully covers a broad range of requirements in confocal and multiphoton imaging with excellent. Science lab, the knowledge portal of leica microsystems, offers scientific research and teaching material on the subjects of microscopy. The leica sp5 is a ultrahigh speed inverted confocal microscope, featuring 5 simultaneous channels, each driven by any of 7 separate laser beams. Microscope imaging software microscope imaging software from leica microsystems combines microscope, digital camera and accessories into one fully integrated solution. Our leica sp5 ii lscm offers an inverted configuration microscope with automatic focus control outfitted with the following objectives.
Introduction to confocal laser scanning microscopy leica this presentation has been put together as a common effort of urs ziegler, anne greet bittermann, mathias hoechli. Rmbf leica sp5 aobs spectral confocal core facilities. Do you struggle with a variety of instruments, protocols, and software interfaces in your lab. This video shows how to configure the internal laser power wavelength etc. Quick operation manual for leica sp5 confocalmultiphoton. The leica sp5 is a pointscan confocal microscope with laser lines at 405, 458, 476, 488, 496, 514, 543, 594, and 633nm. Leica true confocal scanningspectral photometric 5. The leica tcs sp2 system was designed for confocal recording and quantitative measurement via laser point scanning of fluorescence labeled living and fixed. I found out recently through a similar situation that if you have an las installer, the software will work even without a software keydongle. After a short moment you will get the leica application suite start screen.
Leica tcs sp5 confocal louisiana state university health. Leica sp5 inverted confocal manual duke light microscopy. Leica tcs sp5 user manual indiana university bloomington. Confocal microscopes confocal microscopes from leica microsystems are partners in top level biomedical research and surface analysis in material science applications, offering unprecedented precision in threedimensional imaging and exact examination of subcellular structures and dynamic processes. Page 1 queens medical research institute college for medicine and veterinary medicine university of edinburgh manual for the leica sp5c spectral confocal laser scanning microscope version 1.
Leica sp5 inverted confocal microscope with aresonant scanner allowing highspeed confocal imaging and is well suited for livecell imaging and highresolution morphological imaging. Checkin and start up set up acquistion parameters optimize acquistion parameters acquire a zstack sequential scan. Microscopy core facility microscopy service center. Leica las af lite is a platform designed for advanced life science research with leica microsystems confocal and widefield systems.
The facility is equipped with a leica tsc sp5 upright confocal microscope with conventional and resonant scanner, a nikon eclipse te2000e inverted widefield microscope, a zeiss axio scope. What should the user of the laser scanning microscope observe. Instructions for the leica sp5 ii laser scanning confocal microscope. The basic software allows you to capture images from the. Hexagonal packing of nuclei red by microtubules green in coeonocytic female gametophyte of ginkgo biloba. When asked to initialize the stage, select 2 focus your sample and locate the field of. Leica tcs sp5 confocal laser scanning microscope user guide. The leica tcs sp5 confocal fully covers a broad range of requirements in confocal and multiphoton imaging with excellent overall performance. Leica sp5 tile scan protocol 1 open the las af software lower stage completely. It integrates confocal, widefield, stereo, superresolution, and lightsheet instruments from leica microsystems. The leica tcs sp5 is supplied with the latest version of the licensed.
Leica sp5 laser scanning confocal microscope system listing. Fan on turn laser power level to lowest setting min turn argon. Switch speed in an instant the new leica tcs sp5 ii comes with a tandem. With its proven, highefficiency sp detection five channels simultaneously.
Checkin and start up set up acquistion parameters optimize acquistion parameters acquire a zstack sequential scan check out appendix checkin and start up. After the software begins to load youll see text scrolling in the las af start up. Check under configuration that sp5 is selected if not go to configuration and change this to sp5 press ok the confocal. Leica tcs sp5 confocal this is a very flexible and fast confocal system build with a leica dmi 6000 cs inverted, fully automated microscope with motorized stage, condenser, objective and filter turrets. Usermanual for leica sp8 confocal universitetet i bergen. The leica confocal microscope system b46 weill hall revised65 startup turn on 3 red buttons on far right. Fast frame rates, best resolution and depth imaging with full multichannel capabilities in close cooperation with leading research scientists.